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Evaluation of acantholytic activities of immunoaffinity-purified anti-Dsc3, anti-M3AR, and anti-SPCA1 AuAbs in the NMSE model. The extent of acantholysis was measured 24 h after addition to NMSEs of individual or combinations of AuAbs immunoaffinity-purified from IgG fractions pooled from serum of PV patients producing these non-Dsg AuAbs. Each experiment was performed in triplicate. *, p < 0.05 compared with positive control, i.e. total PVIgG, taken as 100%.
