Figure 5.

Evaluation of the effects of immunoaffinity-purified anti-Dsc3, M3AR, and SPCA1 AuAbs on activities of Src, p38 MAPK, and Cs9, CytC release in keratinocytes. Normal human epidermal keratinocytes grown in culture to ∼80% confluence were incubated at 37 °C and 5% CO₂ in the keratinocyte growth medium containing 1.6 mm Ca²⁺ in the presence of equal concentrations of immunoaffinity-purified anti-Dsc3, anti-M3AR, or anti-SPCA1 AuAbs and used to measure CytC release (after 2 h incubation) or the activities of Cs9 (16 h), p38 MAPK (4 h), or Src (1 h). These time points were chosen based on the highest elevation of test parameters in our previous time-course studies (24). The results are expressed as folds of mean control values determined in the keratinocyte monolayers exposed to the same amount of NIgG, taken as 1. #, p > 0.05 compared with control; the rest are p < 0.05.