Figure 3.

Heparin decreases the EC₅₀ value of hIL-12. hIL-12 bioactivity curves showing the IFN-γ production by NK-92MI cells (A), human PBMCs (B), and human T cells (C) exposed to increasing hIL-12 in the presence and absence of heparin (10 μg/ml). The EC₅₀ values of hIL-12 for NK-92MI cells and human PBMCs were significantly decreased by heparin (p < 0.0001 versus hIL-12 alone via extra sum-of-squares F test). All data points represent mean ± S.D. of triplicate measurements. Experiments were performed in triplicate and repeated three times with similar results.