Figure 4.

OGT suppression downregulates EGFR.

Notes: (A) Correlation between OGT expression and EGFR in TCGA datasets. (B) Effect of OGT knockdown on EGFR and PI3K/AKT expression in RCC cells. (C) Interaction between OGT and EGFR in 786-O cells, as determined by co-immunoprecipitation and immunoblot. (D) 786-O cells were treated with sh-NC or sh-OGT followed by EGFR immunoprecipitation with anti-EGFR antibodies; the resulting EGFR immunoprecipitates were assessed by WB for EGFR and O-GlcNAc (RL2) detection.

Abbreviations: Cor, correlation; EGFR, epidermal growth factor receptor; IP, immunoprecipitation; OGT, O-GlcNAc-transferase; O-GlcNAc, O-linked N-acetylglucosamine; NC, negative control; RCC, renal cell carcinoma TCGA, The Cancer Genome Atlas; WB, Western blot.