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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: Nature. 2018 Feb 12;554(7693):549–553. doi: 10.1038/nature25478

Figure 2. KSR1-activated BRAF phosphorylates KSR1-free MEK1.

Figure 2.

a, Putative roles of KSR in RAF-mediated MEK phosphorylation. Model 1 was adapted from reference #7. AS stands for activation segment. b, Y2H binding analysis of WT MEK1 and activation segment mutants MEK1M219V (MV), MEK1N221Y (NY), and MEK1M219W/A220L (MW-AL) to KSR1W831R or BRAFI666R. c, MEK1M219W/A220L (MW-AL), but not WT MEK1, induces KSR1W831R-BRAF dimerization and transactivation as determined by co-IP and pMEK levels. d, MEK1M219W/A220L bound to KSR1W831R induces BRAF activity towards MEK1 free of KSR1. Experiments in b-d were repeated at least 3 times. For gel source data, see Supplementary Fig. 1.