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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Cancer Lett. 2019 Feb 16;449:106–113. doi: 10.1016/j.canlet.2019.02.024

Figure 2. NFX1-123 overexpression enhanced active growth and population doublings in 16E6 HFKs.

Figure 2.

5×105 cells were plated and grown in culture until they no longer doubled within four days. Cumulative population doublings (PD) were calculated. (A) HFKs transduced with FLAG-tagged NFX1-123 (FN123, black hexagon) or an empty vector (LXSN, open diamond) grew for 14 PDs. (B-E) HFKs (HFK2-5) were transduced with 16E6 and then FN123 (16E6/FN123, black square) or LXSN (16E6/LXSN, open circle). Across 4 independent cell lines, 16E6/FN123 HFKs grew for more PDs and total cell numbers than matched 16E6/LXSN HFKs (average difference and 95% CI shown). Horizontal arrows indicate continued active growth of 16E6/FN123 HFKs in culture. Vertical arrows note T1-T4 timepoints for Figures 3 and 5. Asterisks note early and late shared timepoints for Figure 4.