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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Cancer Lett. 2019 Feb 16;449:106–113. doi: 10.1016/j.canlet.2019.02.024

Figure 6. Knock down of NFX1-123 in SiHa cells resulted in a slowed growth rate and decreased hTERT.

Figure 6.

SiHa cells were transduced with either lentivirus containing a short hairpin targeting NFX1-123 (sh1 or sh2) or containing scrambled short hairpin control (scr). (A) NFX1-123 protein levels in sh1 and sh2 cells were decreased at day 6 compared to scr cells at day 3 and day 6 post-transduction. (B) NFX1-91 protein remained constant after short hairpin transduction. A and B: GAPDH used as a loading control (C) For each cell line (scr, sh1, sh2), at both day 3 and day 6, scr cells achieved more growth and had a higher doubling factor than either sh1 or sh2. (D) hTERT mRNA was quantified and values shown were the mean fold change normalized to scr SiHa cells. sh1 and sh2 cells had decreased hTERT compared to scr cells at day 6. hTERT qPCR was normalized to the mRNA levels of the housekeeping gene 36B4, and error bars represent 95% confidence intervals from the technical replicates shown (n = 3).) Doubling factor and hTERT p values: * ≤ 0.05, ** ≤ 0.01, **** ≤ 0.0001