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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Chembiochem. 2018 Nov 12;20(3):312–318. doi: 10.1002/cbic.201800481

Figure 3.

Figure 3.

Application of GlcNAc Electrophilic Probes (GEPs) to characterize OGT-protein substrate recognition. Top: In the presence of protein substrate, GEP1A can label both OGT and its protein substrate, which can be readily detected by click chemistry-based fluorescence assay. The relative level of modified proteins between OGT-WT and its mutants (derived from structure-guided mutagenesis) reflects the altered OGT ability of protein binding compared to sugar binding. Bottom: Preincubation of GEP1 enables OGT crosslinking with various protein substrates in situ.