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. 2019 Feb 28;23(Suppl 1):S11–21. doi: 10.5213/inj.1938040.020

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Copyright © 2019 Korean Continence Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Histological evidence showing 4-vessel occlusion (4-VO) model induces neuronal death in the hippocampal CA1 by 7 days. (A) Time line of ischemic surgery and molecular assay. (B) Representative images of brain sections stained with Nissl and Fluoro-Jade from animals subjected to global ischemia or sham operation 7 days after surgery. (C) Summary data showing the number of living CA1 pyramidal neurons as assessed by Nissl staining (upper), and injured CA1 pyramidal neurons by Fluoro-Jade staining (lower) (n=7 for sham, 4 for ischemic animals). so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum; ML, molecular layer; GL, granular layer; H, hilus; SL, stratum lacunosum-moleculare. ****P<0.0001. Scale bar is 50 μm.