Fig. 4.

Changes of neuroprotective molecules after global ischemia. (A) Wnt family mRNA expression showing time-dependent alterations in ischemia hippocampal CA1 regions as determined by quantitative real time-polymerase chain reaction (qRT-PCR). (B) Growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) mRNA expression quantified by qRT-PCR. (C) Representative immunoblot images of Gadd45b in hippocampal CA1 with complementary quantifications normalized by tubulin (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean ±standard error of the mean (*P <0.05. **P <0.01. ***P <0.001. ****P<0.0001). (D) Confocal imaging depicting Gadd45b (red), counterstained with 4’-6-diamidino-2-phenylindole (DAPI) (blue). Scale bar is 20 µm.