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. 2019 Feb 28;23(Suppl 1):S11–21. doi: 10.5213/inj.1938040.020

Fig. 5.

Fig. 5.

Knockdown of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) accelerates neuronal cell death and mitochondrial dysfunction. (A) Validation of knockdown efficiency for Gadd45b shRNA in cortical neurons by immunoblotting. (B) Summary data of lactate dehydrogenase (LDH) activity assay for cortical neurons treated with 50µM H2O2 (2 hours) in the presence of nontargeting (NT) or Gadd45b shRNA. LDH activity values are normalized to the value of cells exposed to 400μM H2O2 (100%, FK; full kill) after subtracting the value of control cells (0%). (C) Representative immunoblot images showing Bcl-2. (D) Bax expression level in cortical neurons treated with 50µM H2O2 (2 hours) in the presence of NT or Gadd45b shRNA. (E) Quantitative real timepolymerase chain reaction data showing brain-derived neurotrophic factor (BDNF) mRNA expression at indicated time periods after ischemia. (F) Representative immunoblot images Gadd45b in hippocampal CA1 and summary data. (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean±standard error of themean. *P<0.05. **P<0.01. ***P<0.001.