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. 2019 Mar 25;14(3):e0211538. doi: 10.1371/journal.pone.0211538

Fig 3. MICA and MICB in the releasate modulate NKG2D expression and NK cell functions.

Fig 3

(A) To examine the role of MICA and MICB from the platelet releasate (from platelet cloaked tumour cells) in regulating NKG2D and NK cell functions, the platelet releasate was neutralised using monoclonal antibodies against MICA and MICB as previously described. NKG2D expression (A) and NK cell functions (CD107a (B) and IFNgamma (C)) were quantified following standard 4h NK cell function assays by flow cytometry and results are expressed as the mean fluorescent intensity (MFI; A) or as a percentage of unstimulated or IgG control (B and C). (D) Schematic representation of the platelet sNKG2DL mediated decoy mechanism of tumour immune evasion (A-C) Data analysed by ANOVA—each experiment represents mean±S.E.M. of at least three independent experiments. * = p<0.05, ** = p<0.01, *** = p<0.001