Table 2. Average numbers (± SD) of peroxisomes in cells overexpressing MMM1.

strain	Mean ± SD
BY4741 + pFL44	2.62 ± 0.05
BY4741 + pFL44-MMM1	3.13 ± 0.01
BY4741 + OE-MMM1	4.25* ± 0.12
mdm12Δ + pFL44	4.75 ± 0.1
mdm12Δ + pFL44-MMM1	5.70 ± 0.3
mdm12Δ + OE-MMM1	6.55* ± 0.2

Average numbers of peroxisomes per cell observed in cells overexpressing MMM1 from its own promoter (pFL44-MMM1) or from the strong PGK1 promoter (OE-MMM1) on multicopy plasmids in the wild-type and mdm12Δ cells (BY4741 genetic background). Cells were grown on glucose and the average number of peroxisomes presented as mean ± SD. Student’s t test, (*) p-values < 0.05. For each strain, the number of peroxisomes was counted from images of two counts of at least 100 non-budding cells from three independent experiments.