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. 2017 Oct 12;75(8):ftx113. doi: 10.1093/femspd/ftx113

Figure 6.

Figure 6.

OipA is Involved in CagA Translocation from H. pylori 26695 into AGS cells. AGS cells were infected 14 h with H. pylori 26695 rdxA− (oipA phase on), H. pylori 26695/rdxA−/oipA phase off, H. pylori 26695/rdxA−/oipA phase on/cagE− or left uninfected. Adherent H. pylori and AGS cells were subjected to western blotting using either anti phosphotyrosine to visualize translocated CagA in its tyrosine phosphorylated form, anti-CagA, or anti glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. CagA protein is translocated and tyrosine phosphorylated only in the presence of phase on oipA allele and a functional T4SS. Changing the expression phase of oipA to phase off or ablating the cagE gene of the T4SS prevented the translocation of this effector protein. These results are representative of the results obtained in two independent experiments.