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. 2019 Mar 25;14(3):e0214338. doi: 10.1371/journal.pone.0214338

Fig 6. bCAR-mediated transactivation of the proximal promoter and the fragment 3 in CYP3A28 promoter.

Fig 6

The transactivation by bCAR of the most responsive fragments PP and F3 was evaluated. C3A cells were transfected with the control reporter pCMVβ (150 ng/well), each reporter plasmids or PBREM-tk-luc (PBREM, 50 ng/well) and either bCAR expression plasmids or pCI-neo empty vector (25 ng/well). After transfection, cells were treated with vehicle (0.1% DMSO) for 24 hours, and reporter activities were measured. Firefly luciferase activities were normalized with β-galactosidase activities. Data are expressed as mean luciferase activities ± SD (n = 3 or 4). Results shown are representative of 3 independent assays. Statistical significance P < 0.05: *, PP-F3 with empty pCI-neo vs. bCAR.