Table 1. Results from in silico screening of bovine CYP3A28 gene promoter.
| RE- and TF-name | Position in the Promoter Fragments | ||||||
|---|---|---|---|---|---|---|---|
| PP, lnPP | lnPP, F1 | F1 | F2 | F3 | F4 | F5 | |
| CAR/RXRα (c) | / | -876/-851 | / | -4348/-4323 | / | / | -9301/-9277 |
| CAR/RXRα & HNF-4 (c) | / | / | / | / | / | / | -9522/-9498 |
| C/EBPα (c) | / | / | / | -3288/-3273 | -5275/-5260 | -7485/-7471 | / |
| -5645/-5631 | |||||||
| DR-4 (a, b, c) | / | -1250/-1234 | / | / | / | / | / |
| DR-5 (a) | / | / | / | / | -5541/-5525 | / | / |
| ER-6 (a, b, c) | -173/-156 | / | / | / | / | / | / |
| HNF-1 (d) | -196/-179 | / | / | / | / | / | / |
| HNF-3 (c) | / | / | / | / | -6091/-6075 | / | -9313/-9298 |
| -10190/-10174 | |||||||
| HNF-3β (c) | / | / | -1362/-1345 | -4986/-4969 | -6007/-5991 | -7022/-7006 | -10006/-9990 |
| -3003/-2986 | -6540/-6524 | ||||||
| HNF-4 (c, d) | -225/-208 (c, d) | -1143/-1118 (c) | / | -3548/-3523 (c) | -6114/-6090 (d) | -8022/-7998 (c) | / |
| HNF-4α (c) | / | / | / | -4048/-4023 | / | / | / |
| HNF-4 & DR-4 (a, b, c) | / | / | / | / | / | / | -10248/-10244 |
The web-based software NUBIscana and
NHR-scanb were used to identify PXR-RXR and CAR-RXR heterodimers DNA core binding motifs ER6-9 and DR3-5;
MatInspectorc was queried for RXR, HNF-3, HNF-4 and C/EBPα binding sites, while
Matchd through liver specific matrices identified HNF-1, HNF-3 and NHF-4 DNA binding elements.