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The study design: cage layout across 2 rows of an IVC rack and order of applying restraint or tattooing procedures during each week of data recording. The 32 individually numbered mice initially underwent daily acclimation to tail or tunnel handling for 7 d prior to being tattooed or restrained. Eight mice (4 male and 4 female) underwent tattooing or restraint each week, and the sex that was tattooed or restrained was alternated between Tuesdays and Thursdays.
