Figure 5.

(A) Mean Global Mouse Grimace Scale (GMGS) score (all FAU) at each assessment time after each procedure, showing no overall difference between between the restraint (white bars) or tattooed (black bars) groups but more severe grimacing postprocedurally following ear-tagging than following tattooing (Tag compared PP; (P = 0.033). (B) Mice handled by the tail grimaced more severely than those that were tunnel-handled, both before the tattooing or restraint procedures (PA) and at each subsequent time point, including when those mice that were restrained were ear-tagged (P = 0.004). (C) Male mice grimaced more than female mice. (P = 0.004).PA, postacclimation; PP, postprocedure; Tag, after ear tagging.