Table 2.

Matrix effect on MX QC samples in the pooled blank human plasma (n = 5)

MX QC (ng/ml)	PAMX in extracted plasma ± SD (x 105)a	PAMX in mobile phase ± SD (x 105)	MFMX ± SDb	PAIS in extracted plasma ± SD (x 105)	PAIS in mobile phase ± SD (x 105)	MFIS ± SDb	IS Normalized MF ± SDb
3.50	0.40 ± 0.02	0.960 ±0.005	0.42 ± 0.02	5.7 ± 0.2	13.4 ± 0.1	0.42 ± 0.02	1.00 ± 0.07
45.0	6.8 ± 0.3	12.5 ± 0.3	0.54 ± 0.03	7.5 ± 0.4	13.4 ± 0.2	0.56 ± 0.03	0.96 ± 0.07
450	52.0 ± 0.5	101 ± 2	0.51 ± 0.01	6.00 ± 0.09	11.9 ± 0.3	0.50 ± 0.01	1.02 ± 0.03

^aPA = mean peak area

^b$\text{y}\left(\pm {\text{s}}_{\text{y}}\right)=\frac{\text{a}\left(\pm {\text{s}}_{\text{a}}\right)}{\text{b}\left(\pm {\text{s}}_{\text{b}}\right)},{\text{s}}_{\text{y}}=y\cdot \sqrt{{\left(\frac{s_a}{a}\right)}^2+{\left(\frac{s_b}{b}\right)}^2}$