Skip to main content
. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jun 6;901:25–33. doi: 10.1016/j.jchromb.2012.05.036

Table 4.

Matrix effect on MX QCs in six individual lots of blank human plasma matrices (n = 5)

Plasma matrix MX QC (ng/mL) MFMX ± SDa MFIS ± SDa IS normalized MF ± SDa
Lot 1 3.50 1.7 ± 0.1 1.7 ± 0.1 1.0 ± 0.1
45.0 1.5 ± 0.1 1.44 ± 0.09 1.0 ± 0.1
450 1.4 ± 0.1 1.4 ± 0.1 1.0 ± 0.1
Lot 2 3.50 1.27 ± 0.09 1.25 ± 0.08 1.0 ± 0.1
45.0 1.24 ± 0.09 1.23 ± 0.08 1.0 ± 0.1
450 1.3 ± 0.1 1.3 ± 0.1 1.0 ± 0.1
Lot 3 3.50 0.82 ± 0.09 0.80 ± 0.08 1.0 ± 0.1
45.0 0.71 ±0.05 0.71 ± 0.05 1.0 ± 0.1
450 0.74 ± 0.08 0.75 ± 0.08 1.0 ± 0.2
Lot 4 3.50 0.9 ± 0.1 0.9 ± 0.1 1.0 ± 0.2
45.0 0.77 ± 0.09 0.77 ± 0.09 1.0 ± 0.2
450 0.82 ± 0.07 0.82 ± 0.07 1.0 ± 0.1
Lot 5 3.50 0.67 ± 0.08 0.66 ± 0.08 1.0 ± 0.2
45.0 0.71 ± 0.08 0.71 ± 0.07 1.0 ± 0.2
450 0.8 ± 0.1 0.8 ± 0.1 1.0 ± 0.2
Lot 6 3.50 0.84 ± 0.09 0.82 ± 0.08 1.0 ± 0.1
45.0 0.70 ± 0.09 0.70 ± 0.08 1.0 ± 0.2
450 0.75 ± 0.08 0.75 ± 0.08 1.0 ± 0.2
a

MFMX = (mean peak area of MX in the extracted plasma matrix)/(mean peak area of MX in the mobile phase)

b

MFIS = (mean peak area of IS in the extracted plasma matrix)/(mean peak area of IS in the mobile phase)

c

IS normalized MF = MFMX/MFIS.