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. 2017 Sep 1;64(CN Suppl 1):177–181. doi: 10.1093/neuros/nyx227

FIGURE 3.

FIGURE 3.

GPR133 knockdown impairs tumor growth in vitro and in vivo. A, Representative stainings for Ki67 obtained from a patient-derived GBM culture treated with control (scramble) and GPR133 (GPR133-KD) shRNA lentivirus. B, GPR133 knockdown reduced the fraction of Ki67-positive cells in vitro (t-test). C, GPR133 knockdown impaired the clonogenic ability of GBM cells in hypoxia in vitro (t-test). D, Representative microscopic images show that GPR133 knockdown prevented tumor formation in the brain of NOD.SCID mice. The nuclei of tumor cells were stained for human nuclear antigen (hNA). The arrow indicates a few remaining GBM cells bearing the GPR133-KD construct. However, these cells failed to generate a tumor. E, Kaplan–Meier survival curves indicate that GPR133 knockdown prevented death of implanted mice (n = 4 mice/group; logrank test, P < .05). DAPI, nuclear counterstain. Adapted from reference12, published OA-CC-BY-4.0.