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. 2019 Mar 5;8:e43736. doi: 10.7554/eLife.43736

Figure 11. DV cells are replenished by other support cell populations.

(A–B, D–E, G–H) Maximum projections of neuromasts expressing sost:NTR-GFP and sost:nlsEos (A–B), sfrp1a:nlsEos (D–E), and tnfsf10l3:nlsEos (G–H) in the absence of (A, D, G; Mock) or following Mtz-induced DV cell ablation (B, E, H; Mtz). Sost:NTR-GFP cells are shown in green and nlsEos-positive cells are shown in magenta. Arrowheads indicate nlsEos-positive sost:NTR-GFP cells. Scale bar = 10 μm. (C) Percentage of sost:NTR-GFP cells per neuromast labeled by sost:nlsEos following DV cell regeneration. Mock: 97.39 ± 7.14, n = 50 neuromasts (10 fish); Mtz: 56.09 ± 33.72, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001. (F) Percentage of sost:NTR-GFP cells per neuromast labeled by sfrp1a:nlsEos following DV cell regeneration. Mock: 6.15 ± 11.14, n = 50 neuromasts (10 fish); Mtz: 31.27 ± 24.41, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001. (I) Percentage of sost:NTR-GFP cells per neuromast labeled by tnfsf10l3:nlsEos following DV cell regeneration. Mock: 7.31 ± 9.55, n = 50 neuromasts (10 fish); Mtz: 21.11 ± 22.51, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.0004.

Figure 11—source data 1. DV cells are replenished by other support cell populations.
elife-43736-fig11-data1.xlsx (10.2KB, xlsx)
DOI: 10.7554/eLife.43736.036

Figure 11.

Figure 11—figure supplement 1. Ablation of DV cells does not decrease the number of other support cell populations.

Figure 11—figure supplement 1.

(A) Total number of sost:nlsEos-positive support cells per neuromast following DV cell regeneration. Mock: 21.42 ± 3.29, n = 50 neuromasts (10 fish); Mtz: 12.70 ± 3.35, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001. (B) Total number of sfrp1a:nlsEos-positive support cells per neuromast following DV cell regeneration. Mock: 12.00 ± 2.54, n = 50 neuromasts (10 fish); Mtz: 11.32 ± 2.71, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.2413. (C) Total number of tnfsf10l3:nlsEos-positive support cells per neuromast following DV cell regeneration. Mock: 18.40 ± 2.44, n = 50 neuromasts (10 fish); Mtz: 17.46 ± 2.49, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.0699.
Figure 11—figure supplement 1—source data 1. Ablation of DV cells does not decrease the number of other support cell populations.
elife-43736-fig11-figsupp1-data1.xlsx (10.7KB, xlsx)
DOI: 10.7554/eLife.43736.033
Figure 11—figure supplement 2. Expression of nlsEos transgenes does not alter number of sost:NTR-GFP cells.

Figure 11—figure supplement 2.

Total number of sost:NTR-GFP cells per neuromast in sost:nlsEos (sost), sfrp1a:nlsEos (sfrp1a), and tnfsf10l3:nlsEos (tnfsf10l3) fish. Sost: 7.08 ± 1.59, n = 50 neuromasts (10 fish); Sfrp1a: 6.80 ± 1.92, n = 50 neuromasts (10 fish); Tnfsf10l3: 6.96 ± 1.70, n = 50 neuromasts (10 fish); mean ± SD; Kruskal-Wallis test, Dunn’s post-test, p>0.9999 (all comparisons).
Figure 11—figure supplement 2—source data 1. Expression of nlsEos transgenes does not alter number of sost:NTR-GFP cells.
elife-43736-fig11-figsupp2-data1.xlsx (9.7KB, xlsx)
DOI: 10.7554/eLife.43736.035