Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information | ||
|---|---|---|---|---|---|---|
| Strain, strain background (Danio rerio) |
sfrp1a:nlsEos | This paper | Tg[sfrp1a:nlsEos]w217; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
sfrp1a:GFP | This paper | Tg[sfrp1a:GFP]w222; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
tnfsf10l3:nlsEos | This paper | Tg[tnfsf10l3:nlsEos]w218; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
tnfsf10l3:GFP | This paper | Tg[tnfsf10l3:GFP]w223; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
sost:nlsEos | This paper | Tg[sost:nlsEos]w215; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
sost:NTR-GFP | This paper | Tg[sost:NTR-GFP]w216; CRISPR-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
myo6:mKate2 | This paper | Tg[myosin6b:mKate2]w232; Gateway cloning and Tol2-mediated transgenesis |
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| Strain, strain background (Danio rerio) |
brn3c:GFP | PMID: 15930106 | ZFIN ID: ZDB-TGCONSTRCT-070117–142 | Tg[Brn3c:GAP43-GFP]s356t | ||
| Strain, strain background (Danio rerio) |
myo6:GFP | PMID: 27991862 | ZFIN ID: ZDB-TGCONSTRCT-170321–2 | Tg[myosin6b:GFP]w186 | ||
| Antibody | anti-Parvalbumin (mouse monoclonal) |
Millipore Sigma | MAB1572 | (1:500) | ||
| Antibody | anti-GFP (rabbit monoclonal) |
Thermo Fisher Scientific |
ABfinity G10362 | (1:500) | ||
| Antibody | anti-BrdU (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
DHSB: G3G4; RRID: AB_2314035 | (1:100) | ||
| Recombinant DNA reagent |
pBSK mbait-GFP | PMID: 25293390 | ||||
| Recombinant DNA reagent |
mbait-nlsEos | This paper | pDEST vector expressing nuclear-localized Eos following the mbait-HSP sequence from pBSK mbait-GFP; see Materials and methods for more info. |
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| Recombinant DNA reagent |
pBSK mbait-NTR-GFP | This paper | Modified pBSK mbait-GFP vector expressing enhanced potency nitroreductase fused to GFP; generated via Gibson assembly |
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| peptide, recombinant protein |
Cas9 protein with NLS (injection ready) |
PNA Bio | PNA Bio: CP02 | |||
| Commercial assay or kit |
NucleoSpin Plasmid | Machery-Nagel | 740588.25 | |||
| Commercial assay or kit |
NucleoSpin Gel and PCR Clean-up |
Machery-Nagel | 740609.25 | |||
| Commercial assay or kit |
Plasmid Maxi | Qiagen | 12163 | |||
| Commercial assay or kit |
RNA Clean and Concentrator |
Zymo Research | RCC-25 | |||
| Chemical compound, drug |
Gateway BP Clonase II | Thermo Fisher Scientific |
11789020 | |||
| Chemical compound, drug |
Gateway LR Clonase II | Thermo Fisher Scientific |
11791020 | |||
| Chemical compound, drug |
5x Isothermal Reaction Buffer |
PMID: 19363495 | ||||
| Chemical compound, drug |
Metronidazole | Sigma-Aldrich | Sigma-Aldrich: M1547 | |||
| Chemical compound, drug |
LY411575 | Sigma-Aldrich | Sigma-Aldrich: SML0506 | |||
| Chemical compound, drug |
Neomycin | Sigma-Aldrich | Sigma-Aldrich: N1142 | |||
| Chemical compound, drug |
f-ara-EdU | Sigma-Aldrich | Sigma-Aldrich: T511293 | |||
| Chemical compound, drug |
BrdU | Sigma-Aldrich | Sigma-Aldrich: B5002 | |||
| Chemical compound, drug |
FM 1-43FX | Molecular Probes | Molecular Probes: F35355 | |||
| Software, algorithm |
GraphPad Prism | GraphPad Software | www.graphpad.com | |||
| Software, algorithm |
Slidebook | Intelligent Imaging Innovations (3i) |
www.intellgent-imaging.com | |||
| Software, algorithm |
Zen Black | Zeiss | www.zeiss.com | |||
| Software, algorithm |
FIJI | PMID: 22743772 | ||||