| Denature | 95 °C | 5 min |
| Incubation | 60 °C | 25 min |
| Denaturation | 95 °C | 5 min |
| Incubation | 60 °C | 85 min |
| Denaturation | 95 °C | 5 min |
| Incubation | 60 °C | 175 min |
| Hold | 4 °C |
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