| Denature | 95 °C | 2 min |
| *Denature | 95 °C | 30 s |
| *Annealing | 58 °C | 30 s |
| *Extension | 72 °C | 30 s (repeat * for 25 cycles) |
| Elongation | 72 °C | 5 min |
| Hold | 4 °C |
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