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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Transplantation. 2019 Apr;103(4):705–715. doi: 10.1097/TP.0000000000002495

Figure 3. Effect of rapamycin and everolimus on the extracellular acidification rates of Treg cells.

Figure 3.

(A) The extracellular acidification rates (ECAR, mpH/min) were measured in Treg cells expanded 5 days in the presence of 100nM of rapamycin (closed circles) or everolimus (open circles), and compared with 5 day-expanded untreated Treg cells (Control, closed squares) and conventional T cells (Tconv, closed triangles) cultured in the same Treg cell expansion medium. (B-E) The ECAR parameters were generated with the Seahorse XF Glycolysis Stress Test Report Generator and are shown as follows: (B) Baseline ECAR, (C) Glycolysis, (D) Maximal glycolytic capacity -Glycol. Capacity-, and (E) Glycolytic reserve -Glycol. Reserve-. Data is showing as the mean ± SD pooled from at least three independent (n=3) experiments and five replicates per condition in each experiment. *p<0.05 and **p<0.001 indicate significant pairwise differences in Wilcoxon test following Kruskal–Wallis ANOVA test. NS indicate no significant differences among treatments.