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Fluoronitrobenzene reagents for protein lysine modification. (A) Labeling of bovine insulin with 1-fluoro-2-nitro-4-trimethylammoniumbenzene iodide improved the hydrophilicity of the proteinand made it readily crystallizable. (B) PEGylation of proteins using a PEGylated ester of 4-fluoro-3-nitrobenzoic acid.
