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. 2019 Feb;31(1):203–211. doi: 10.21147/j.issn.1000-9604.2019.01.15

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Copyright © 2019 Chinese Journal of Cancer Research. All rights reserved.

This work is licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Stromal interaction molecule 1 (STIM1) expression level in osteosarcoma cells and tissue specimens. (A) Western analysis. Cytoplasmic extracts from osteosarcoma cells (143B, U2OS, Saos-2, KHOS and MG63) and human osteoblast (HOB 1 and HOB 2) cells were analyzed by Western blot using anti-STIM1 and anti-glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) antibodies as described in methods. Quantitation was performed by densitometry and normalized to GAPDH expression. Data are presented as from three independent experiments. *, P<0.05, compared with HOB 1 and HOB 2; (B) Representative immunohistochemical staining of STIM1 in osteosarcoma using tissue microarrays. The lower panel is a magnified view of a region in the respective image in the upper panel. Scale bars are 200 μm in the upper panel and 50 μm in the lower panel; (C) Overall survival of patients in STIM1 high and STIM1 low groups (P=0.028).

Inline graphic — Stromal interaction molecule 1 (STIM1) expression level in osteosarcoma cells and tissue specimens. (A) Western analysis. Cytoplasmic extracts from osteosarcoma cells (143B, U2OS, Saos-2, KHOS and MG63) and human osteoblast (HOB 1 and HOB 2) cells were analyzed by Western blot using anti-STIM1 and anti-glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) antibodies as described in methods. Quantitation was performed by densitometry and normalized to GAPDH expression. Data are presented as from three independent experiments. *, P<0.05, compared with HOB 1 and HOB 2; (B) Representative immunohistochemical staining of STIM1 in osteosarcoma using tissue microarrays. The lower panel is a magnified view of a region in the respective image in the upper panel. Scale bars are 200 μm in the upper panel and 50 μm in the lower panel; (C) Overall survival of patients in STIM1 high and STIM1 low groups (P=0.028).