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. 2019 Feb;31(1):203–211. doi: 10.21147/j.issn.1000-9604.2019.01.15

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Copyright © 2019 Chinese Journal of Cancer Research. All rights reserved.

This work is licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Effect of store-operated calcium entry (SOCE) inhibitor and stromal interaction molecule 1 (STIM1) siRNA on osteosarcoma cell migration. (A) Wound healing assays. Micrographs of 143B and U2OS cells treated with SKF96365 (20 μmol/L) for 12 h. The migration distance is presented relative to that of control (absence of SKF96365) in each cell line; (B) Cell viability. 143B and U2OS osteosarcoma cells treated with SKF96365 (20 μmol/L) for 12 h and analyzed by MTS assays; (C) Transwell assays and quantitation of migrated cells in 143B and U2OS cultures treated with SKF96365 (20 μmol/L) for 16 h; (D) Transwell assays and quantitation of migrated cells in 143B and U2OS cells treated with control or STIM1 siRNAs for 16 h. The number of migrated cells is presented relative to that of control siRNA. Data are presented as from three independent experiments. *, P<0.05; NC, negative control.

Inline graphic — Effect of store-operated calcium entry (SOCE) inhibitor and stromal interaction molecule 1 (STIM1) siRNA on osteosarcoma cell migration. (A) Wound healing assays. Micrographs of 143B and U2OS cells treated with SKF96365 (20 μmol/L) for 12 h. The migration distance is presented relative to that of control (absence of SKF96365) in each cell line; (B) Cell viability. 143B and U2OS osteosarcoma cells treated with SKF96365 (20 μmol/L) for 12 h and analyzed by MTS assays; (C) Transwell assays and quantitation of migrated cells in 143B and U2OS cultures treated with SKF96365 (20 μmol/L) for 16 h; (D) Transwell assays and quantitation of migrated cells in 143B and U2OS cells treated with control or STIM1 siRNAs for 16 h. The number of migrated cells is presented relative to that of control siRNA. Data are presented as from three independent experiments. *, P<0.05; NC, negative control.