Figure 1.

In vitro platelet aggregation responses are not affected by incubation with NRTIs. Platelet aggregation was monitored using PRP in a microplate assay. PRP was treated with plasma C_max levels of NRTIs, or their respective anabolite, for 30 min at 37°C. Maximum aggregation is reported in responses to increasing concentrations of ADP (A), collagen (B), or TRAP6 (C). (a) Platelets were treated with 3 μg·ml⁻¹ ABC or vehicle control (1% DMSO). (b) Aggregation was recorded following treatment with 0.22‐μg·ml⁻¹ TAF, 15‐ng·ml⁻¹ TFV, or vehicle control (1% DMSO). (c) Platelets were incubated with 0.33‐μg·ml⁻¹ TDF, 0.25‐μg·ml⁻¹ TFV, or vehicle control (2% DMSO). Data are representative of a five independent experiments for each condition. *P < 0.05, significantly different from vehicle; two‐way ANOVA