Figure 2.

Abacavir enhances collagen‐evoked granule release. Flow cytometry was used to assess real‐time changes in surface expression of platelet granule markers. PRP was diluted 1:200 into physiological buffer containing fluorescently conjugated CD62P, CD63 and PAC‐1 antibodies. Stable baselines were achieved in the first 30 s of sample acquisition and arrowheads (A) indicate when collagen (10 μg·ml⁻¹) was added to the samples. Addition of collagen led to a rapid and sustained increase of CD62P (a), CD63 (b), and PAC‐1 (c) binding. Responses were monitored in the presence of vehicle control (0.02% DMSO), ABC (3 μg·ml⁻¹), TAF (0.22 μg·ml⁻¹), or TDF (0.33 μg·ml⁻¹). Data are representative of six independent experiments. *P < 0.05, significantly different from vehicle; two‐way ANOVA