Figure 4.

Abacavir enhances collagen‐evoked platelet aggregation in vivo. Platelet aggregation responses were monitored in vivo using terminally anaesthetized mice. Mouse platelets were isolated, radiolabelled (1.8‐MBq indium tropolone), and transfused into a recipient mouse. ABC (a; 30 μg·ml⁻¹), TDF (b; 3.3 μg·ml⁻¹), or TAF (c; 2.2 μg·ml⁻¹) was administered i.p. for 30 min. A bolus of 50 μg·kg⁻¹ collagen was injected (arrows), and responses were monitored for 10 min. ABC and TDF experiments were conducted using wild‐type (WT) C57bl/6 mice, whilst Ces1c ^−/− mice were utilized for TAF. The AUC was calculated for the duration of the experiment and representative traces and summary data are shown. Data are representative of seven independent experiments. *P < 0.05, significantly different from vehicle; paired, two tailed Students' t‐test