Figure 2.
Betulinic acid improves glucose‐lipid homeostasis in DIO C57BL/6J mice. Obese C57BL/6J mice were fed a HFD in the presence and absence of betulinic acid (100 mg·kg−1·day−1) for 6 weeks. (a) Body weight during the 6‐week treatment. (b) Curve for amount of food consumed. (c) Fasting blood glucose. (d) I.p. glucose tolerance test (IPGTT). The mice were fasted overnight, and glucose was injected i.p. (1 g·kg−1 body weight), and blood glucose levels were determined at the indicated time points. (e) I.p. insulin tolerance test (IPITT). The insulin was injected i.p. (0.75 U·kg−1 body weight), and blood glucose levels were determined at the indicated time points. (f) Serum TC, TG, low‐density lipoprotein cholesterol (LDL‐c), HDL‐c levels. (g, h) TG and TC in faeces were extracted and determined. Data are presented as means ± SEM (n = 7). P < 0.05, HF versus chow (*) or HF versus betulinic acid (#) group, NS: no significance