Figure 4.

Dioscin decreased the expression levels of TIGAR in vitro and in vivo. (a) Effects of dioscin on the mRNA levels of TIGAR in vitro and in vivo based on real‐time PCR assay. Total RNA samples were obtained from SMMC7721 and HepG2 cells, livers of rats, and tumour tissues of nude mice. (b) Effects of dioscin on the protein levels of TIGAR in vitro and in vivo based on Western blotting assay. Total proteins from SMMC7721 and HepG2 cells, livers of rats, and tumour tissues of nude mice were isolated. After separation on SDS‐PAGE gels, the proteins were detected and imaged. All data are expressed as mean ± SD (n = 5). *P < 0.05 versus control group