Figure 6.

TIGAR siRNA aggravated the effects of dioscin on TIGAR‐mediated signal in vitro. (a) Effects of dioscin on colony formation, motility, cell apoptosis, and DNA damage after TIGAR‐siRNA transfection in SMMC7721 cells. SMMC7721 cells were cultured in six‐well plates and transfected with the siRNA liposomes in antibiotic‐free cell medium. Colony formation, motility, cell apoptosis, and DNA damage were detected after 24 hr of transfection in the absence or presence of dioscin (5.6 μM). (b) Effects of dioscin on the expression levels of TIGAR, p53, p‐Akt/Akt, p‐mTOR/mTOR, CDK5, and p‐ATM/ATM in SMMC7721 cells after TIGAR‐siRNA transfection in SMMC7721 cells. All data are expressed as mean ± SD (n = 5). *P < 0.05 versus control group; n.s: no significance