Figure 7.

dNK cells and dMA express VIP receptors, and it regulates their functional profile. (a) 5 × 10⁵ dNK cells or (b) 5 × 10⁵ dMA were cultured in RPMI 0% FCS in the absence or presence of VIP (10 or 100 nM). (I) Cells were harvested and used for RT‐qPCR. Each point depicted represents an independent sample. Results are expressed as mean ± SEM of A.U. (gene/YWHAZ)*10². Friedman's test and Dunn's test were used to compare the results (n = 8). (II) The supernatants were used for the BioPlex assay. The results are shown as mean ± SEM. Mann–Whitney test with a post hoc Dunn's test were used to compare the results (n = 9). dMA: decidual macrophages; dNK: decidual natural killer; FCS: fetal calf serum; VIP: vasoactive intestinal peptide