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. 2019 Jan 31;23(4):2558–2567. doi: 10.1111/jcmm.14146

Figure 3.

Figure 3

FBXL10 suppressed HG‐induced production of ROS, inflammation and apoptosis in H9c2 cells via PKC β2. (A) H9c2 cells were transfected with FBXL10. Indicated protein level was detected by Western blotting at indicated time point and normalized to β‐actin. Data represent the mean ± SD of three independent experiments. ***P < 0.001; **P < 0.01. (B) H9c2 cells transfected with FBXL10 were treated with HG with or without Ly333531. ROS was analyzed by DCFH‐DA. Data represent the mean ± SD of three independent experiments. *P < 0.05. (C) H9c2 cells transfected with FBXL10 were treated with HG with or without Ly333531. Protein level of P67phox were analyzed by Western blotting and normalized to β‐actin. Data represent the mean ± SD of three independent experiments. **P < 0.01. (D) H9c2 cells transfected with FBXL10 were treated with HG with or without Ly333531. The secretion of TNF‐α, IL‐1β and IL‐6 was determined by ELISA. Data represent the mean ± SD of three independent experiments. **P < 0.01. (E) H9c2 cells transfected with FBXL10 were treated with HG with or without Ly333531. Apoptosis was analyzed by Flow cytometry. Data represent the mean ± SD of three independent experiments. **P < 0.01