Figure 6.

Autophagy attenuates cell apoptosis by inhibiting CHOP up‐regulation in gly‐HDL‐treated RAW264.7 cells. RAW264.7 cells were exposed to gly‐HDL (100 mg/L) in the presence or absence of rapamycin (1 μmol/L) or 3‐MA (3 mmol/L) for 24 h. (A) The protein levels of beclin‐1 and LC3 were analysed by Western blotting. Cell viability; (B) and apoptosis (C) were measured by MTT assay and flow cytometry, respectively. D, The protein levels of p‐PERK and CHOP were analysed by Western blotting. Cells were transfected with beclin‐1 siRNA, treated with 100 mg/L gly‐HDL for 24 h, and then beclin‐1 and CHOP levels; (E) and cell apoptosis; (F) were measured by Western blotting and flow cytometry, respectively. Data are expressed as the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01 vs control group; ^# P < 0.05 vs gly‐HDL group