Figure 5.

A, Expression profile of 44 cytokines detected by Luminex assay in human vaginal tissue from post‐menopausal women with severe POP. B, Comparative expression of 14 cytokine proteins in the LET‐treated group (black bars) and non‐treated group (control, white bars). Concentration of chemokines was determined in 500 μg of vaginal tissue lysates. A significant difference is indicated by * (P < 0.05), ** (P < 0.01), or *** (P < 0.001). C, ELISA analysis of IL‐1RA expression (pg/mL). No significant difference was detected between the two groups (P = 0.34). D, Analysis of cytokine gene expression between LET‐treated (black bars) and non‐treated (control, white bars) vaginal tissue samples. The expression of cytokine genes was normalized to three housekeeping genes: TBP, YWHAZ, SDHA. Statistical analysis was performed with independent sample t test. Data presented as mean ± SEM, N = 12