Figure 4.

MB cell adaptation to long‐term JPH treatment does not cause resistance. A, The expression levels of CD98 and LAT1 in HD‐MB03 and DAOY cells chronically exposed for 120 d to the indicated concentrations of JPH203 were analysed by immunoblot. Tubulin was used as a loading control. B, Proliferation assay of HD‐MB03 and DAOY cells treated with 20 or 30 µmol/L of JPH203 or DMSO (control, Ct) for 120 d. C, Viability assay of HD‐MB03 and DAOY cells treated with 20 or 30 µmol/L of JPH203 or DMSO (control, Ct) for 120 d (***P < 0.001; **P < 0.01; *P < 0.05; ns: P > 0.05, Student's t test)