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. 2019 Feb 2;23(4):2619–2631. doi: 10.1111/jcmm.14156

Figure 5.

Figure 5

Role of N‐cadherin in the transendothelial migration of tumour cells in vitro. (A) CellTracker Red CMTPX‐stained A2058 melanoma cells were seeded onto a confluent monolayer of D3 cells and left for 5 hours. Representative immunofluorescence images are shown. The two bottom panels (i and ii) are higher magnifications of the respective sectors in the top image. (B) CellTracker Red CMTPX‐stained MDA‐MB‐231 cells were seeded onto a confluent monolayer of D3 cells and left for 5 hours. Representative immunofluorescence images are shown. The two bottom panels (iii and iv) are higher magnifications of the respective sectors in the top image. (C) MDA‐MB‐231 or 4T1 breast cancer or A2058 or B16/F10 melanoma cells were seeded onto confluent monolayers of RBECs and left for 5 hours. Protein samples were collected from mono‐cultures or co‐cultures (mixed cells). Representative Western blot shows expression of N‐cadherin in brain endothelial and melanoma cells, but not in breast cancer cells (MDA‐MB‐231 and 4T1). EC = endothelial cell