Figure 6.

Role of N‐cadherin in the transendothelial migration of breast cancer cells in vivo. 4T1 mouse triple negative breast cancer cells expressing tdTomato red fluorescent protein were injected into mice expressing Venus‐YFP in endothelial cells. Mice were killed after 5 or 12 days (A and B, respectively). Representative confocal micrographs show that 4T1 breast cancer cells are N‐cadherin negative and metastasize efficiently to the brain. (A) Transmigrating cell in day 5 after inoculation of tumour cells (indicated by white arrow). (B) Already formed metastatic lesions in day 12. (a) nuclei (Hoechst 33342 staining). (b) endothelial cells (Venus‐YFP). (c) tdTomato‐4T1 cells. (d) N‐cadherin staining. (e) merged image of (b) and (d). (f) merged image of (a)‐(d). (g) merged image of (c) and (d)