Figure 1.

Confirmation of MAFF knockdown in PHM1‐31 myometrial cells. PHM1‐31cells were transduced with shRNA (NTC, sh1 or sh2) and MAFF knockdown analysed in the presence or absence of IL1B (10 ng/mL). (A) MAFF knockdown at the transcript level was quantified by quantitative PCR after no‐, 1, 3 or 8 h of IL1B treatment. (B) MAFF knockdown at the protein level was detected by immunoblot by using antibodies against MAFF or actin to control for protein loading after no‐ or 3 h of IL1B treatment. Data represent mean values ± SEM; unpaired t‐test; *P < 0.05; **P < 0.01; ***P < 0.001, ΔΔΔ P < 0.001. Asteriks correspond to the significance with the control (NTC) at the same time point and triangles to the significance between controls