Figure 4.

Binding of MAFF transcription factor to CXCL1 loci in PHM1‐31 myometrial cells. (A) Location of MAFF binding sites at the CXCL1 loci were established using the UCSC genome browser database and data from the ENCODE consortium (PMID: 22955616), SYDH track (GEO:GSM935306) showing MAFF specific binding to CXCL1 loci in HEPG2 cells. Chosen peaks for CXCL1 (A‐C) are marked in yellow, rectangles on top of peaks represent statistically significant peak calls, the darker rectangles being more significant. (B) ChIP analysis of MAFF transcription factor binding to CXCL1 loci. PHM1‐31 cells were treated (or not) with 10 ng/mL IL1B for 3 h and analysed by chromatin immunoprecipitation using antibodies against MAFF or FLAG to control for non‐specific binding. Data represent mean values ± SEM; unpaired t‐test; *P < 0.05; **P < 0.01; ***P < 0.001