Figure 2.

Enhancing GATA5 expression inhibits growth and colony formation in HCC cells. A, Semi‐quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) was applied to analyse the effect of the expression of mRNA of GATA5 while transfected with siRNA‐GATA5 vectors in HCC cells. B, HLE, Bel 7402 and PLC/PRF/5 cell were transfected with CDH empty vector, CDH‑GATA5, siRNA‐GATA5 or siRNA‐scramble for 72 h, and the growth of the cells was measured by MTT assays. C, HLE, Bel 7402 and PLC/PRF/5 cell were transfected with CDH‐GATA5, siRNA‐GATA5 or no transfect (control cells) for 72 h, and colony formation was observed by optical microscopy (100×). P < 0.05 indicates statistical significance