Figure 4.

Influence of GATA5 on the expression of MMP2 and MMP9 in HCC cells. A, HLE, Bel 7402 and PLC/PRF/5 cells were transfected with CDH empty vector, CDH‐GATA5 or no transfect (control cells) for 48 h. Western blot analysis of the expression of MMP2 and MMP9 in HLE, Bel 7402 and PLC/PRF/5 cells. B and C, HLE, Bel 7402 and PLC/PRF/5 cell were transfected with pcDNA3.1‐GATA5 or no transfect (control cells) and were then cultured at 37°C in a humidified atmosphere of 5% CO₂. Localization of MMP2 and MMP9 was visualized, and images were captured under laser confocal microscopy. Nuclei are stained with DAPI (blue). MMP2 and MMP9 are labelled with FRITC (red). D, The invasion of the cells was detected while transfected with GATA5 expressed vectors or siRNA vectors in HCC cells. Three independent experiments were performed for these data