Table 1.
Relative quantification of gene expression measured by qRT-PCR of selected genes in seeds of Quadrato D’Asti giallo incubated for 4 days on control medium and media with different concentrations of ABA or NaCl.
Control | ABA 1 μM | ABA 5 μM | ABA 10 μM | NaCI 25 mM | NaCI 50 mM | NaCI 100 mM | |
---|---|---|---|---|---|---|---|
CaPYL4 | 1 | 1.561 ± 0.014 | 1.787 ± 0.005 | 1.855 ± 0.005* | 1.267 ± 0.004 | 1.558 ± 0.005 | 1.603 ± 0.005 |
CaPYL8 | 1 | 0.850 ± 0.030 | 1.093 ± 0.014 | 1.492 ± 0.027 | 2.278 ± 0.013*** | 2.216 ± 0.011*** | 1.778 ± 0.007*** |
CaABI | 1 | 0.751 ± 0.001 | 0.756 ± 0.001 | 1.177 ± 0.001 | 0.873 ± 0.001 | 0.960 ± 0.001 | 1.282 ± 0.001 |
CaAHGl | 1 | 1.652 ± 0.009* | 0.770 ± 0.005 | 0.827 ± 0.003 | 0.866 ± 0.001 | 0.760 ± 0.001 | 0.990 ± 0.001 |
CaHAI | 1 | 0.331 ± 0.001* | 0.592 ± 0.003 | 3.142 ± 0.004*** | 2.546 ± 0.002* | 3.786 ± 0.005* | 4.567 ± 0.004*** |
CaSnRK2.3 | 1 | 0.851 ± 0.007 | 0.844 ± 0.017 | 1.486 ± 0.018* | 1.071 ± 0.010 | 1.321 ± 0.009** | 1.340 ± 0.014* |
CaSnRK2.4 | 1 | 0.921 ± 0.009 | 1.401 ± 0.006 | 1.870 ± 0.010* | 2.085 ± 0.011* | 1.785 ± 0.008* | 2.176 ± 0.010** |
CaSnRK2.6 | 1 | 1.371 ± 0.003 | 3.269 ± 0.004** | 3.706 ± 0.003*** | 3.169 ± 0.003** | 2.971 ± 0.002*** | 5.211 ± 0.006*** |
All data are expressed relative to control treatment as mean ± SD (n = 4). Asterisks represent significance levels using Student t-test; ∗P ≤ 0.05; ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.005.