Figure 4.

Anacardic acid (AA) attenuates the overexpression of cardiac hypertrophy‐linked genes in transverse aortic constriction (TAC) mice. (A) Q‐PCR analyses were used to determine the mRNA expression of MEF2A. (B) ChIP‐PCR assays were used to identify the regulatory relationships among MEF2A, atrial natriuretic peptide (ANP) and myosin heavy chain (β‐MHC), which clearly showed the binding of MEF2A at the promoter region of ANP and β‐MHC, but MEF2A did not bind to the promoter region of α‐actin. (C and D) Q‐PCR analyses of the mRNA levels of ANP and β‐MHC in the mouse heart tissues. (E and F) Western blots showing the expression of ANP and β‐MHC at the translational level in the mouse hearts. (G and H) Haematoxylin and eosin staining of cardiac tissue in the mouse hearts. (I) The cross‐sectional area of cardiomyocytes. *P < 0.05 vs the normal group. ^# P < 0.05 vs the TAC group (n = 6)