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. 2019 Mar 19;10:501. doi: 10.3389/fimmu.2019.00501

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Copyright © 2019 Duan, Chen, Razavi, Wei, Tao, Rao and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased neutrophil recruitment in α_2BAR-Tg mice after MSU stimulation. Wt and α_2BAR-Tg mice were treated with MSU to induce peritonitis. After 6 h, the total PECs were harvested. (A) Quantitative analysis of total number of PECs. (B) The PECs were stained with anti-CD11b, anti-F4/80, and anti-Ly6G antibodies. CD11b⁺ cells were gated for the analysis of macrophage (CD11b⁺Ly6G⁻F4/80⁺) and neutrophil (CD11b⁺Ly6G⁺F4/80⁻). Representative images were shown. (C) Statistical bar graph showing the percentages of macrophages and neutrophils in total CD11b⁺ cells. (D) The absolute numbers of neutrophils and macrophages in PECs. Data are represented as mean ± SEM (n = 6–8/group). The results shown are from one of three independent experiments. **p < 0.01, ***p < 0.001. ns, not significant.