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. 2019 Mar 19;10:501. doi: 10.3389/fimmu.2019.00501

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Copyright © 2019 Duan, Chen, Razavi, Wei, Tao, Rao and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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α_2BAR over-expression does not affect the development of macrophage and neutrophil. The PECs and bone marrow cells were collected from untreated α_2BAR-Tg and Wt mice. (A,B) Percentage of CD11b⁺F4/80⁺ macrophages in PECs. The cells were stained with anti-CD11b, anti-F4/80, and anti-Gr1 antibodies. The CD11b+ cells were gated for analysis. The percentage and absolute number of CD11b⁺F4/80⁺ macrophages and CD11b⁺Gr1⁺F4/80⁻ neutrophils in PECs were presented. (C,D) Bone marrow cells were stained with anti-CD11b, anti-F4/80, and anti-Ly6G antibodies. The percentage and absolute number of CD11b⁺Ly6G⁺F4/80⁻ neutrophils in bone marrow cells was shown. The results shown are from one of three independent experiments (n = 6–8/group).